Lab equipment and available techniques

Laboratory equipment and available techniques

Our research group has a wide experience in the study of platelet function and coagulation anomalies related to thrombotic and bleeding disorders. The laboratory has complete equipment for investigating complex clinical situations complementing standard hemostasis routine diagnostic tests, as well as for basic and translational research projects.

Flow Cytometry

• BD Accuri C6 Flow Cytometer equipped with a blue and red laser, two light scatter detectors, and four fluorescence detectors.

We developed flow cytometric methods to study platelet activation and procoagulant activity, especially cytosolic ion fluxes and protein phosphorylations.

• Gallios Flow Cytometer (10 Colors, 3 Lasers); in common with other hematological research labs

Thrombin generation assays

• Calibrated Automated Thrombogram (CAT) (Stago) performed with fluoroskan Ascent instrument (Thermo Scientific)
• Thrombodynamics Analyzer (Hemacore)
• ST Genesia (Stago)

We use global hemostasis tests to investigate the correlation of parameters describing thrombin generation, clotting formation and fybrinolisis with phenotypes of patients presenting different state of hemostasis (from patients anticoagulated with DOACs to patients with rare bleeding disorders).

Microscopy

• Fluorescent inverted microscope Nikon Eclipse Ti2

We aim to implement analysis of platelets with fluorescent microscopy coupled with a flow-based system.

Specific hematological and haemostasis assays

• Aggregometer APACT 4004 (Endotell)
• Sysmex (XP-300, Sysmex DIGITANA AG)

Spectrophotometers

• Multimode microplate reader Spark 20M (TECAN)
• Fluoroskan Ascent (Thermo Scientific)
• Spectrophotometer (NanoDrop OneC, Thermo Scientific)

Protein analysis

• Electrophoresis and blotting systems (SDS-PAGE, Bio-Rad)
• Imager (LAS 500, GE HealthCare)